16 MOLECULAR BIOLOGY ClearLine deoxyribonucleotides (dNTPs), PCR grade water and enhancer Part no. Designation Concentration Packaging € Deoxyribonucleotides 257743 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 25 mM of each 100 mM 8 tubes of 0.5 ml NC - 257744 Mix of dNTPs, solutions of dATP, dCTP, dGTP and DTTp, 10 mM of each 40 mM 2 tubes of 0.5 ml NC - 257746 Set of dNTP, solution of dATP, dCTP, dGTP and DTTp, 100 µM, in separate tubes 100 mM 16 tubes of 0.25 ml NC - 257745 DNTP set, solution of dATP, dCTP, dGTP and DTTp, 25µM, in separate tubes 100 mM 4 tubes of 0.25 ml NC - MgCl2 257741 MgCl2 25 mM 25 mM 10 tubes of 1.5 ml NC - 257742 MgCl2 25 mM 25 mM 3 tubes of 1.5 ml NC - Enhancer PCR 257543 Betaine solution enhancer for complex matrices 5 M 5 tubes of 1 ml NC - PCR grade water 257605 PCR grade ultrapure water - 1 bottle of 1000 ml NC - 257606 PCR grade ultrapure water - 1 bottle of 500 ml NC - 257607 PCR grade ultrapure water - 6 tubes of 5 ml NC - Part no. Designation Concentration Packaging € 257757 10X standard buffer, 1.7mM MgCl2 for Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257749 10X ammonium buffer, 1.5 mM MgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257750 10X combined buffer, 1.5 mMMgCl2 Hot Disco Taq and Disco Taq DNA polymerase 10X 3 tubes of 1.5 ml NC - 257755 GC-rich matrix buffer 1 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - 257756 GC-rich matrix buffer 2 4X Hot Disco Taq DNA polymerase 4X 10 tubes of 1.5 ml NC - Buffers for Disco Taq and Hot Disco Taq DNA polymerase Hi-fi Pop DNA polymerase 2 U/µl, with 5X buffer and MgCl2 z Hi-fi Pop DNA polymerase 2 U/µl, with 5X Hi-fi buffer and 25 mM MgCl2 z Amplification of up to 18kb of gDNA and 25kb of DNA λ z To be completed with dNTPs, matrix, primers and water Hi-fi Pop DNA polymerase z For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis z 60 times more accurate than conventional Taq polymerase z Amplification of long, GC-rich DNA sequences z Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional) z High elongation rate: 10 sec/kb z 3’ –› 5' exonuclease activity Hi-fi Pop DNA polymerase master mix 2X, ready to use z Master mix ready to use, addition of matrix, primers and water only z Amplification of up to 11 kb of gDNA z The 2X buffer contains Hi-fi Taq DNA polymerase, dNTPs, Hi-fi buffer and 3 mM MgCl2 for a final concentration of 1.5 mM Designation No. of units / no. of 50 µl reactions 5X Hi-fi buffer and 25 mM MgCl2 € Hi-fi Pop DNA polymerase 2 U/µl 100 257651 NC - 500 257653 NC - 2500 257650 NC - 5000 257652 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Part no. € Hi-fi Pop DNA polymerase Master mix 2X 100 250 257654 NC - 500 1250 257656 NC - 2500 6250 257655 NC - 5000 12500 257657 NC -
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