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Depuis 2017, le consortium BIORCELL3D® regroupe des chercheurs en Biologie et Pharmaco-chimie de l’équipe de Recherche IMoST (UMR INSERM-UCA 1240) et des spécialistes de l'industrie des Biotechnologies issus des sociétés BIOPASS et BIOMARQUEURS (Clermont-Ferrand - 63). Ensemble, ils développent et commercialisent des OUTILS DE BIOTECHNOLOGIE INNOVANTS APPLIQUÉS À LA CANCÉROLOGIE ET À LA SANTÉ HUMAINE, tels que:

  • Artificial Tumours® (3D cultures) for new cancer drugs screening. These preclinical models are derived from well-known tumour cell lines that are widely used by researchers and that faithfully reproduce avascular micro-tumours. They are available as a service or as ready-to-use kits (Opti3D® kits).
  • Calf serum-free culture media to optimise 3D cultures and allow the preservation of biological samples over several days without freezing (OptiPASS® media range).
  • Fluorescent reagents capable of measuring the level of resistance of tumour cells following anticancer treatments in a rapid and dynamic manner (Patent registered LightSpot® by UCA-BIOMARQUEURS).
  • Services: custom development of new 3D models, screening of molecules (drug screening) or efficacy tests using artificial tumours.


The products

Artificial Tumor Kits® in ready-to-use microplates for in vitro testing.

Artificial tumors® (3D cultures) from tumor cell lines widely used in oncology, and in particular derived from aggressive triple-negative breast cancers.

  • Artificial tumors more closely mimic the microenvironment of avascular tumors in vivo than 2D cultures
  • These models are increasingly becoming part of the drug development processes.

Discover
Complete, ready-to-use serum-free cell culture media.

OptiPASS® media used for monolayer (2D) culture of tumour or healthy cells and for 3D culture of a wide range of tumour types of pre-clinical/clinical interest.

Three applications:

Bioproduction-2D culture

For the 2D culture of VERO E6 cells, the OptiPASS® medium (OPTI04) allows:

  • Cell proliferation and viability similar to serum medium culture (reference
  • Preservation of cell phenotype

(Requires prior coating of culture plates)

Oncology- 3D culture

Examples of 3D cultures with OptiPASS® medium: tumour lines validated in 2D and 3D cultures:

  • Breast: MDA-MB-231, SUM1315, BT474
  • Prostate: PC-3
  • Lung : NCI-H1975
  • Ovary: SK-OV-3

For artificial tumour cultures®, the OptiPASS® medium (OPTI03) allows:

  • Maintaining / increasing the rate of proliferation / growth
  • Maintenance of metabolic gradients within cultures (live cells/dead cells)
  • Reproducibility of production in terms of size and proliferation/growth
Oncology-preservation

Once produced, the 3D cultures can be stored for several days in OptiPASS® (OPTI05), under anoxic conditions and at 4°C before being analysed. Examples of tumour lines validated with this method: breast: MDA-MB-231, SUM1315.


Discover

Innovation improving the detection, localisation and quantification of MultiDrug Resistance proteins in all cellular samples.
Applications:

  • Better control of efflux: kidney, liver, blood-brain barrier, placental barrier, etc
  • Evaluate cellular resistance to different molecules and exogenous agents (active ingredients, pollutants, anti-infectious agents)

Aims:

  • Optimising cancer treatment by quantifying chemoresistance of certain drugs
  • Improving efflux modulation

Available for different excitation/emission wavelengths.



Service provision

Development of new 3D tumour cell models on demand.
  • Analysis of requirements according to the cell type and choice of the most suitable 3D technique
  • Identification of optimal growing conditions:
    • Choice of culture device (specific microplates)
    • Cell concentrations, type and matrix concentration (if applicable)
    • Objective: reproducibility and stability of models (coefficient of variation in size <10%)
  • Characterisation of models:
    • Growth profile
    • Metabolic profile
    • Maximum cultivation time
    • Topology (Scanning Electron Microscopy SEM)
    • Ultrastructure (Transmission Electron Microscopy TEM)
    • Expression profile of proteins of interest
  • Deliverables:
    • 3D cell culture protocol adapted to your cell type
    • Ready-to-use kits containing your 3D models (if development possible in OptiPASS®)
  • Estimated development time: between 2 and 3 months depending on the models
Development of new 3D tumour cell models on demand.
  • Requirement analysis
  • Choice of tumour models
  • Development of adapted tests (when appropriate)
  • Identification of treatment modalities / duration / type of exposure / doses
  • Reception and preparation of test molecules
  • Carrying out the tests
  • Identification of deliverables (non-exhaustive)
    • Viability %, Growth inhibition, IC50,
    • Impact on the cell cycle
    • Tagging and quantification of targets of interest
    • Clonogenic tests, etc.
  • Secure archiving of raw and analysed data
  • Deliverables: full analysis reports
  • Deliverables: full analysis reports




Documentation and videos

Presentation of the screening service

For more details on Biocell3D® products and services, brochures are available for download.

LightSpot        Artificial Tumour Kits® and serum-free culture media



Romain GUEGAN

Head of cell culture and therapy projects in France

Port. 07 63 62 19 98
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