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Molecular biology - Proteins Sample preparation Protease inhibitors

Protease inhibitors

Protease Inhibitor Mix

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• Suited to protect proteins during insulation processes from bacteria, fungi, yeast, plant and mammalian cells or for general applications
• Lyophilized, sufficient to protect 100 ml of cell lysate

Whatman blotting paper

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Nytran N nylon blotting membrane

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• Suitable for applications that require a lower charge
• Designed for Southern and Northern blotting, colony and plaque lifts and Dot-/Slot-Blots
• Compatible with isotopic and nonisotopic detection methods
• Excellent signal-to-noise ratios
• Moderate positive charge
• Consistent membrane morphology
• High sensitivity
• Nytran binding capacity: >400 µg/ cm²
• It is available in 0.2 µm and 0.45 µm pore sizes for optimal retention of oligos and larger DNA fragments

Nytran SuPerCharge (SPC) nylon blotting membrane

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• Very high positive charge, low background
• Nytran SuperCharge membrane binds nine times more molecules than a conventional nylon membrane
• The great nylon density (compared to typical nylon membranes) provides increased binding sites for your samples
• Excellent sy mmetry: gives the membrane the ability to lie flat
• Very uniform pore size and pore distribution compared to typical nylon membranes: lead to greater reproducibility of results across a membrane and from blot to blot

Amersham™ HYBOND blotting membrane

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Hybond-N
• Strong, supported membrane
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Recommended for radioactive blotting
• Nucleic acids can be crosslinked to nylon using UV ligh

Hybond-N+
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• For use with radioactive or nonradioactive chemiluminescence and chemifluorescence detection systems

Hybond-NX
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Developed specifically for use with low hybridization buffer volumes and high-throughput applications
• Gives clean backgrounds

Hybond-XL
• For nucleic acids blotting
• Binding capacity up to 600 µg/ cm²
• Efficient binding of small fragments
• Positively charged nylon membrane which is designed to give optimum signal-to-noise ratios when used with radioactively labeled probes

More dimensions available: please contact us.

Amersham Protran nitrocellulose blotting membrane

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The Hybond and Whatman brands combine to give Amersham optimised blotting membranes.

Amersham Hybond PVDF blotting membrane

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• Ready-to-use solution for blotting applications
• Contains precut Amersham Hybond P 0.2 µm PVDF membranes preassembled with 2 x 3 mm Chr Blotting papers
• The orientation of the sandwich must be so that the membrane is on the anode side of the gel
• 3 mm Chr paper sheets cover each side of the sandwich
• The gel is ready for transfer

Protran NC nitrocellulose blotting membrane

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• 100% pure nitrocellulose membrane 0.1 µm for molecules<7 kD
• High binding, low background
• High retention of small proteins:
• 0.2 µm membrane retains samples of less than 20 KD
• 0.45 µm membrane is designed for molecules over 20 KD
• Excellent stability of proteins on the Protran (5 years)
• No pre-wetting step with methanol: before the transfer, a simple wetting with water is enough
• Excellent stability of the membrane: allows many manipulations

HRP anti-IGG antibody, Amersham

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• For Western blotting
• Optimised for use in conjunction with Amersham ECL detection reagents
• Antibodies to IgG conjugated to horseradish peroxidase
• Classical form (full antibody) or F(ab')2 fragment
• F(ab')2 fragment form eliminates aspecific interactions of the Fc moiety, facilitates cell penetration, no interference with anti-Fc antibodies

Labeling and detection for protein / Western Blotting / secondary antibodies

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Amersham ECL and MP Hyperfilm™

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For detection of the chemiluminescence signal of the nucleic acid and protein blots.

Amersham ECL™, ECL Start™, ECL Advance™, ECL Select™, ECL Prime

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Cytiva AlkPhos direct labeling and detection system

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The system is based on the direct labeling of DNA or RNA probes with thermostable alkaline phosphatase. The labeled probes are then hybridized with the target.

Amersham Hyperscreen intensifying screens

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Offer rapid results and high sensitivity for detecting32Pand125I in Southern, Northern, and Western blots

QuickStain staining kit for Proteins (Cy5) quantification

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• Allows fluorescence detection of Proteins directly after SDS-PAGE or transfer
• Excitation and emission wavelength:: 650 nm / 670 nm
• Up to 150 samples
• qualitative or quantitative marking according to staining time
• Does not require discolouration step
• Compatible with pre-cast gels
• Compatible with chemiluminescence or fluorescent secondary marking
• Without epitopes saturation for western blotting
• Low background noise