Cell culture - Serums - Media Greiner Bio-One 3D cell culturing - Magnetic suspensions and bioprinting 3D cell culture - Spheroids - Organoids: M3D Greiner Bio-One Kit

3D cell culture - Spheroids - Organoids: M3D Greiner Bio-One Kit

System for 3D cell culture thanks to the transient magnetization of cells that naturally attach to the NanoShuttle-PL magnetic beads.
Thanks to the magnetic force, the cells are ready for either magnetic levitation or spheroid printing, to form 3D in vitro representation models.

• in vitro reproduction of the native tissue environment
• Versatile system compatible with all cell types even in co-culture
• 3D model formation in a few hours after incubation with magnetic beads
• Allows cell co-culture
• No impact of the magnetized beads on the cells (proliferation, viability, phenotype ...)
• Centered positioning of the spheroids
• No specialized equipment, no media, no artificial substrate
• Easy to handle, no sample loss

Selection guide - Greiner Bio-One M3D cell culture system

Application note - Interest of M3D in automated 3D culture systems

Application note Multi-Magpen Greiner Bio-One - magnetic trasnfer of 3D cell cultures Access to Greiner Bio-One publications

Source : Greiner Bio-One

Variants and accessories

Magnetic levitation

Spheroid printing

Magnetic ring printing and imaging system

Magnetic NanoShuttle-PL

Accessory

Multi-MagPen 24 and 96 wells

Accessories

Transiently and non-invasively magnetized cells are placed in a Cell-Repellent plate. A magnet placed above the plate attracts the cells to the air/medium interface. Magnetic forces bundle the cells together, favouring intercellular interactions at the origin of the extracellular matrix synthesis.

Transiently and non-invasively magnetized cells are placed in a plate and a magnetic holder will be positioned underneath that plate, keeping the cells at the bottom of the well. In a few minutes or even hours, the plate containing the spheroids can be removed from the magnetic holder and cultured over the long term.

Based on the 3D magnetic Spheroid printing, transiently and non-invasively magnetized cells thanks to Nanoshuttle-PL beads are positioned in a ring shape mimicking certain in-vivo patterns and their functions (ex. contraction).

• Nano-particles assembly (50 nm diameter), does not bind any specific receptors, works with all cell types - attaches to the plasma membrane electrostatically
• Consists of biocompatible gold, iron oxide and Poly-L-Lysine
• No effects on proliferation, viability, metablism, oxydative stress, phenotype
• Does not disturb experimental techniques such as immunohistological labelling, fluorescence detection, HR microscopy, Western Blot, PCR/qPCR ...

Magnetic tool for the individual manipulation of formed spheroids

• Fast, easy and simultaneous transfer of multiple 3D cell cultures without pipetting
• Complete change of media by a simple pick-up-and-drop step
• Simplified co-culture of different cell types
• Ideal for facilitating immunohistochemical staining, blocking and washing of spheroids

Specific terms
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