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Molecular biology Complementary products of protein biology Acrylamide gels Preparation

Acrylamide gels Preparation

Acrylamide / bisacrylamide 19: 1

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Ready to use solution 40%. Acrylamide / bis - acrylamide consists of 38% acrylamide and bis - acrylamide 2%. The solution is optimised for electrophoresis of nucleic acids.

Acrylamide / Bisacrylamide 37.5:1

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Ready to use solution 40% (38.96% acrylamide, 1.04% bisacrylamide). This concentration makes the solution an ideal choice for the resolution of high molecular weight protein separation.

Acrylamide / Bis - Acrylamide 19:1

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Ready-to-use solution 40%. Acrylamide / bisacrylamide consists of 38% acrylamide (v) and 2% bis (acrylic acid) (v).

Acrylamide / Bis - Acrylamide 37.5:1

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Ready-to-use solution 40% (acrylamide 38.96%, bis-acrylamide 1.04%). This concentration makes the solution an ideal choice for solving small macromolecules.

Agarose for protein electrophoresis

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Ammonium persulphate

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Initiates the polymerization of acrylamide gels. Used with TEMED.

Tween® 20

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Blocking agent. Nonionic detergent used in solutions.

Ammonium persulphate

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Ammonium persulphate initiates the polymerization of the acrylamide gels. Used with TEMED.

Alternatives to alkylphenol ethoxylate detergents

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• Alkylphenol ethoxylates are included in Annex XIV of REACH program due to their toxicity
• Several detergents in the Triton X and Tergitol X range are among the targeted molecules
• Merck offers a range of alternative detergents

ECOSURF EH detergents:
• Ethoxylated substitutes
• Excellent wetting and solid surface cleaner
• Low odour, easily biodegradable and very low toxicity to the aquatic environment

ECOSURF SA detergents:
• Ethoxylated substitutes
• Easier handling, low pour point and fast dissolution
• Low odour, hydrophobic, good detergency, biorenewable base, easily biodegradable and very low toxicity to the aquatic environment

TERGITOL 15-S detergents:
• Substitutes based on secondary alcohols
• Excellent wetting, fast dissolving and rapid reduction of surface tension
• Low odour, good detergency and easily biodegradable

TERGITOL TMN detergents:
• Substitute based on branched secondary alcohol ethoxylates
• Very good wetting, emulsifying and detergent properties
• Very low surface tension
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