Nucleases -

DNase I (RNase-free)

• Endonuclease non-specifically degrading single and double stranded DNA by releasing di-, tri- and phosphorylated oligonucleotides in 5'.
• Used to remove contaminant DNA from RNA preparations prior to RT-PCR and RT-qPCR applications
• Also used to remove the DNA matrix from the reaction medium following in vitro transcription
• Available in 1000 and 5000 unit formats

Exonuclease I

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• Catalyses the removal of nucleotides from single-stranded DNA in the 3' -> 5' direction
• Does not degrade double-stranded DNA or RNA
• Ideal for single-strand primers degradation after PCR prior to DNA sequencing or SNP analysis
• Used for single-stranded DNA degradation in a double-stranded DNA preparation
• Active in a wide variety of reaction buffers
• Available in 4000 and 20000 units formats

Illustra Shrimp Alkaline Phosphatase and Exonuclease I

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Use alkaline phosphatase and exonuclease to clean PCR reactions before sequencing.

T7 Endonucléase I

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• It is structure-selective enzyme that recoginzes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA structures, Holliday structures or junctions and more slowly, nicked dsDNA
• The cleavage site is the first, second or third phosphodiester bond that is 5' to the mismatch
• Ultrapure recombinant enzyme

Possible applications:
• Recognition of mismatched DNA in particular in the context of genome editing using the Crispr method
• Resolve four-way junction or branched DNA
• Detect or cleave heteroduplex and nicked DNA
• Available in 250 and 1250 unit format