Molecular biology - Proteins Marking and detection - Western blotting Transfer membranes

Transfer membranes

Amersham Protran nitrocellulose blotting membrane

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The Hybond and Whatman brands combine to give Amersham optimised blotting membranes.

Amersham Hybond PVDF blotting membrane

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• Ready-to-use solution for blotting applications
• Contains precut Amersham Hybond P 0.2 µm PVDF membranes preassembled with 2 x 3 mm Chr Blotting papers
• The orientation of the sandwich must be so that the membrane is on the anode side of the gel
• 3 mm Chr paper sheets cover each side of the sandwich
• The gel is ready for transfer

Protran NC nitrocellulose blotting membrane

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• 100% pure nitrocellulose membrane 0.1 µm for molecules<7 kD
• High binding, low background
• High retention of small proteins:
• 0.2 µm membrane retains samples of less than 20 KD
• 0.45 µm membrane is designed for molecules over 20 KD
• Excellent stability of proteins on the Protran (5 years)
• No pre-wetting step with methanol: before the transfer, a simple wetting with water is enough
• Excellent stability of the membrane: allows many manipulations

Immobilon transfer membranes

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