Molecular Biology - Nucleic Acids PCR in end point Standard Routine DNA polymerases Hi-fi Pop DNA polymerase

Hi-fi Pop DNA polymerase

• For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis
• 60 times higher fidelity than conventional Taq polymerase
• Amplification of long, GC-rich DNA sequences
• Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional)
• High elongation rate: 10 sec/kb
• 3' -' 5' exonuclease activity

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Variants and accessories

Hi-fi Pop DNA polymerase 5 U/µl, with 5X buffer and MgCl₂

Hi-fi Pop DNA polymerase master mix 2X, µready to use

Enhancer for complex matrices

• Hi-fi Pop DNA polymerase 2 U/µl, with 5X Hi-fi buffer and 25 mM MgCl₂
• Amplification jusqu'à 18kb d'ADNg et 25 kb d'ADN λ
• To be completed with dNTPs, matrix, primers and water

• Ready-to-use master mix, addition of template, primers and water only
• Amplification of up to 11 kb of gDNA
• Master Mix 2Xcontains Hi-fi Taq DNA polymerase, dNTPs, Hi-fi buffer and 3 mM MgCl2 for a final concentration of 1.5 mM

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