Molecular biology Specialty ranges of molecular biology reagents EURx (cloning) T7 Endonucléase I

T7 Endonucléase I

• It is structure-selective enzyme that recoginzes and cleaves mismatched DNA, heteroduplex DNA, cruciform DNA structures, Holliday structures or junctions and more slowly, nicked dsDNA
• The cleavage site is the first, second or third phosphodiester bond that is 5' to the mismatch
• Ultrapure recombinant enzyme

Possible applications:
• Recognition of mismatched DNA in particular in the context of genome editing using the Crispr method
• Resolve four-way junction or branched DNA
• Detect or cleave heteroduplex and nicked DNA
• Available in 250 and 1250 unit format

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Specific terms
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