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Disco taq DNA polymerase

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• High throughput and reliable amplification for routine applications, screening or high-throughput screening
• Amplification jusqu'à 5 kb, compatible avec le TA-cloning et l'incorporation de dUTP
• 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity
See specific terms

Variants and accessories

Disco taq 5 U/µlDisco taq and Disco taq Fever master mix 2XDisco Taq without glycerol
Disco taq 5 U/µl, with or without buffer and MgCl2:
• Disco taq polymérase 5 U/µl, avec ou sans tampon 10X et MgCl2
• To be completed with dNTPs, template, primers, water, 10X buffer and MgCl2 if not supplied
• Several buffers available
Disco taq and Disco taq Fever master mix 2X, ready to use:
• Master mix prêt à l'emploi, ajout de la matrice, des amorces et de l'eau uniquement
• The Disco taq 2X master mix contains Disco taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 at a final concentration of 1.5 or 2 mM, and stabilising agents
• Le master mix Disco taq Fever 2X est fourni avec un tampon Fever, pour des amplifications jusqu'à 4kb avec une spécificité accrue, même avec les templates difficiles

Disponible en version «ready to load», contenant un tampon de charge et un colorant rouge pour suivre la migration :
• Allows direct deposition after PCR, on agarose or SDS gel
• No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp
Disco Taq without glycerol:

• Disco taq 5 U/µl or 50 U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2
• Pour les applications impliquants une étape de lyophilisation, ou nécessitant des pipetages précis de faibles volumes (manuels ou via automate de pipetage)
• 1U allows an average of 500 reactions to be carried out
• Several buffers available

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Specific terms
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