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Hot Disco Taq DNA polymerase

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• Inactivated at room temperature, activation at 95°C for 15 min
• Incorporation possible de dUTP
• High specificity and sensitivity for high performance
• Pour détection de cibles même en faible quantité, le screening, le mutliplexage, la PCR directe sur colonies, et les applications de qPCR

Samples available, please contact us!
See specific terms

Variants and accessories

Hot Disco Taq 5U/µlHot Disco Taq master mix 2XHot Disco Taq GC master mix (2X)Glycerol-free Hot Disco TaqHot Disco Taq master mix (5X) lyophilised
Hot Disco Taq 5U/µl, with or without buffer and MgCl2:
• Hot Disco Taq polymérase 5 U/µl, avec ou sans tampon 10X et MgCl2
• To be completed with dNTPs, template, primers, water, 10X buffer and MgCl2 if not supplied
• Several buffers available
Hot Disco Taq master mix 2X, ready to use:
• Master mix prêt à l'emploi, ajout de la matrice, des amorces et de l'eau uniquementt
• The 2X master mix contains Hot Disco Taq DNA polymerase, dNTPs, MgCl2 at a final concentration of 1.5 mM, and buffer 1 (KCI/(NH4)2 SO4 Tris) or buffer 2 ((NH4)2 SO4)

Available in a "Ready to load" version, containing a loading buffer and a red dye to monitor migration:
• Possibilité de dépôt directement après PCR, sur gel d'agarose ou SDS
• There is no interference with PCR, and the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp
Hot Disco Taq master mix 2X, ready to use:
• Master mix prêt à l'emploi, ajout de la matrice, des amorces et de l'eau uniquement
• For the amplification of GC-rich DNA sequences
• Minimise le risque d'artefact dû à une polymérisation aspécifique possible avant la première étape de dénaturation
• The 2X master mix contains Hot Disco Taq DNA polymerase, optimised buffer, dNTPs, 3.0 mM MgCl2 and stabilising agents

Available with master mix I or II. :
• Le master mix I convient dans la plupart des cas. Si néanmoins les résultats obtenus ne sont pas satisfaisants, il est recommandé alors d'utiliser le master mix 2
• Contact us to obtain samples so that we can select the right master mix to optimise your results
Glycerol-free Hot Disco Taq, with or without 10X buffer:
• Disco Taq 5U/µl avec tampon de stockage sans glycérol, avec ou sans tampon 10X et MgCl2 25 mM
• For applications involving a freeze-drying step, or requiring precise pipetting of small volumes (manual or via automated pipetting) or automated high-throughput screening
• Several buffers available
Hot Disco Taq master mix (5X), lyophilised

• Ready to use once reconstituted in the buffer supplied, with only the matrix, primers and water added
• Stable 12 mois à température ambiante : stokage et transport à température ambiante
• Amplification up to 4 kb
• La Hot Disco Taq master mix (5X) reconstituée, est composée de la Hot Disco Taq DNA polymérase, des dNTPs, du tampon NH4+, et du MgCl2
• Available in a "Ready to load" version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for monitoring migration

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Specific terms
The sales prices displayed on this page are expressed without tax, those valid at the time of consultation, and take precedence over any price visible in a paper catalogue or in a PDF document.
The indication of the stock is not contractual: this data is in constant evolution and can vary according to the desired quantities.
* Items marked "Consult us for delivery time" are only available on order, and are neither exchangeable nor refundable.
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