Molecular Biology - Nucleic Acids Cloning and expression In vitro expression kit RTS in vitro protein expression kit

RTS in vitro protein expression kit

Variants and accessories

RTS 500 ProteoMaster in vitro protein expression kit (5 reactions)

RTS 9000 E. coli in vitro protein expression kit (1 reaction)

RTS 100 E. coli disulfide in vitro protein expression kit (5 and 24 reactions)

• Designed for protein expression in yields of 0.5-6 mg in a 1 ml reaction volume
• Reaction time of 6 to 24 hours thanks to CECF (Continuous-Exchange Cell-Free) technology
• Proteins synthesis of 8.5 to 120 kDa
• Contains the reagents required for 5 x 1 ml transcription-translation reactions
• Contains a control vector encoding chloramphenicol acetyltransferase
• Compatible with the Eppendorf Thermomixer C

• Designed for protein expression in yields of 5-50 mg in a 10 ml reaction volume
• Proteins synthesis of 15 to 120 kDa
• Reaction time of 6 to 24 hours thanks to CECF (Continuous-Exchange Cell-Free) technology
• Contains the reagents required for 1 x 10 ml transcription-translation reaction
• Contains a control vector encoding chloramphenicol acetyltransferase
• Initially designed to express proteins in sufficient quantities for diagnostic tests, NMR/X-ray analysis or toxicological studies
• Expression products can also be used as antigens and for functionality tests
• Compatible with the Eppendorf Thermomixer C

• Allows the synthesis of soluble proteins with their dissulphide bridges
• Rapid optimization of expression conditions (chaperones, detergents, temperature, duration, etc.)
• Facilitated testing of different constructions
• Expression of genes of toxic product
• Proteins synthesis of 10 - 120 kDa
• Protein synthesis of 10 - 120 kDa hRTS 100: includes components to perform 24 x 50 µl transcription-translation reactions, yield up to 80 µg
• Protein synthesis of 10 - 120 kDa RTS 500: includes components to perform 5 x 1 ml transcription-translation reactions, yield up to 2.5 mg

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Specific terms
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