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BioreactiveReagent for molecular biologyProtein extraction and purification

28-4017-48

28-4017-48 · GSTrap 4B, volume: 5 x 5 ml

Name: GSTrap 4B, volume: 5 x 5 ml
Brand: Cytiva
SKU: 28-4017-48
Price: 1340.00 EUR

Made in Europe

Sales Unit: 1

Brand: Cytiva

Part Number: 28401748

NACRES Code: NA.56

Delivery time on request

1 340.00 €

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GSTrap columns are designed for one-step purification of GST-tagged proteins. The columns are prepacked with the matrices:
• Glutathion Sepharose HP: for high-resolution purifications, from a more concentrated sample;
• Glutathion Sepharose FF: for high flow rate purifications;
• Glutathion Sepharose 4B, standard support.

Available in 2 formats: 1 or 5 ml column.

Suggested application(s)Proteomics and protein analysis

Volume5 ml

Gross weight0,05 kg

Gross dimensions21,00 x 11,00 x 3,00 cm

Customs Code3921190099

UNSPSC Code41106500

28-4017-48 - Safety data

28-4017-48 - Manufacturer data sheet GE chromatography guide Can't find the technical documentation you need? Click here to contact our technical hotline

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GST fusion protein cleavage enzyme

Used for site-specific separation of the GST tag from proteins:
• expressed using pGEX-T vectors: Thrombin Protease;
• expressed using pGEX-X vectors: Facto Xa Protease;
• expressed using pGEX-6P vectors: PreScission™ Protease.

PreScission Protease: one unit will cleave >90 % of 100 µg of a test GST-fusion protein in 50 mm Tris-HCl, 150 mm NaCl, 1 mm EDTA, 1 mm DTT, pH 7.0 at 5 °C for 16 h.
Thrombin Protease: one unit of enzyme cleaves 90% of 100 μg of a test GST fusion protein when incubated in 1 × PBS at 22 °C for 16 h
Factor Xa Protease: one unit cleaves >90 % of 100 μg of a test GST fusion protein when incubated in 1 mm CaCl₂, 100 mm NaCl, and 50 mm Tris-HCl (pH 8.0) at 22 °C for 16 h.

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