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BioreactiveReagent for molecular biologyAmplification reagent for molecular biology
523585

523585 · FastGene® Scriptase II ReadyMix 5X with random hexamers, oligo(dT) primers, RNase inhibitor and accessory protein, 100 reactions

Sales Unit: 1
Brand: Nippon genetics
Part Number: LS65
NACRES Code: NA.55
Delivery time on request
Shipping cost with ice pack
6.90 €
391.00 €
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Three reverse transcriptases suited to different cDNA synthesis requirements

• M-MLV–derived reverse transcriptases for cDNA synthesis from RNA
• Available as reverse transcriptase with reagents, cDNA synthesis kit, or 5X ReadyMix, depending on the range

Additional information:

Sample profile: Low amounts of RNA, synthesis of long cDNA
Activity temperature: 37–42 °C (ReadyMix Scriptase II: reverse transcription at 42 °C only)
Maximum cDNA length: ≈12 kb
Applications: RT-PCR; cDNA synthesis for NGS library preparation

Available in four formats:
- Scriptase II cDNA synthesis reagents: reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
- ReadyMix 5X: contains random hexamers, an RNase inhibitor and an auxiliary protein; add RNA and RNase-free water
- ReadyMix 5X with oligo(dT): same composition with additional oligo(dT) primers; add RNA and RNase-free water
Suggested application(s)PCR and nucleic acid amplification
Gross weight0,00 kg
Gross dimensions13,00 x 9,00 x 1,50 cm
Customs Code3507909090
UNSPSC Code41106300
en523585 - Manufacturer data sheeten523585 - Safety dataCan't find the technical documentation you need? Click here to contact our technical hotline

Variant(s)

Sample profile: High amounts of RNA; low-complexity matrices
Activity temperature: 37–42 °C
Maximum cDNA length: ≈5 kb
Applications: end-point RT-PCR

Available in two formats:
- Scriptase Basic cDNA synthesis reagents: reverse transcriptase with 10X buffer, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- Basic cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added

Available in stock: {0}Supply in progressDelivery time on request
523345 · FastGene® Scriptase Basic, enzyme with 10X buffer, dNTPs and RNase-free water, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
Special delivery
523349 · FastGene® Scriptase Basic cDNA synthesis kit with 10X buffer, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
Special delivery

Sample profile: Low amounts of RNA, synthesis of long cDNA
Activity temperature: 37–42 °C (ReadyMix Scriptase II: reverse transcription at 42 °C only)
Maximum cDNA length: ≈12 kb
Applications: RT-PCR; cDNA synthesis for NGS library preparation

Available in four formats:
- Scriptase II cDNA synthesis reagents: reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
- ReadyMix 5X: contains random hexamers, an RNase inhibitor and an auxiliary protein; add RNA and RNase-free water
- ReadyMix 5X with oligo(dT): same composition with additional oligo(dT) primers; add RNA and RNase-free water

Available in stock: {0}Supply in progressDelivery time on request
523347 · FastGene® Scriptase II reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
Special delivery
523351 · FastGene® Scriptase II cDNA synthesis kit with 5X buffer, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
Special delivery
523403 · FastGene® Scriptase II ReadyMix 5X with random hexamers, RNase inhibitor and auxiliary protein, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
Special delivery

Sample profile: RNA with complex secondary structures and low abundance, enabled by enhanced thermostability
Activity temperature: 37 – ≈55 °C
Maximum cDNA length: ≈12 kb
Applications: RT-qPCR; first-strand cDNA synthesis for NGS and cDNA library construction

Available in three formats:
- Scriptase III cDNA synthesis reagents: reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor supplied separately
- cDNA synthesis kit: reverse transcriptase, 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor; only RNA sample needs to be added
- 5X ReadyMix: reverse transcriptase, RT buffer, dNTPs and RNase inhibitor premixed; oligo(dT) primers and random hexamers provided separately to be added with the RNA and required water

Available in stock: {0}Supply in progressDelivery time on request
523645 · FastGene® Scriptase III, reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
523646 · FastGene® Scriptase III, cDNA synthesis kit with 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request
523647 · FastGene® Scriptase III ReadyMix 5X with RT buffer, dNTPs, RNase inhibitor; oligo(dT) and random hexamer primers supplied separately, 100 reactions
Sales Unit: 1
Nippon genetics
Delivery time on request

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