NimaGen : What reagents and consumables for the séquençage NGS and Sanger?

NimaGen, based in the Netherlands, develops molecular biology reagents that save laboratories valuable time. A pioneer of RC-PCR, the brand offers EasySeq kits for targeted enrichment and sequencing of viral genomes or gene panels, with a single-tube workflow. It complements this with proven Sanger/CE consumables — BrightDye, ExS-Pure, NimaPOP polymer, capillaries — compatible with Applied Biosystems platforms. A reliable, cost-effective solution for research, surveillance and quality control, from the benchtop to high-throughput.

A Dutch innovator turned industry benchmark

Founded in the early 2010s in Nijmegen (Netherlands), NimaGen first made its mark with a complete portfolio for Sanger sequencing and fragment analysis: BrightDye cycle-sequencing kits, ExS-Pure purification reagent, and ABI-compatible capillary consumables. Building on that hands-on expertise, the company then moved into NGS with a distinctive approach: RC-PCR, which brings indexing and library preparation together in a single PCR. This technology propelled the EasySeq kits into HLA typing, targeted panels and viral genomics applications, widely adopted during the pandemic. Backed by academic and hospital collaborations and an international distribution network, NimaGen stays true to the same ethos: simplicity, reproducibility and outstanding value for money.

Expertise and areas of specialisation

At NimaGen, expertise revolves around two complementary pillars.

- In targeted NGS, the brand offers EasySeq RC-PCR kits that drastically cut hands-on steps. Dual-indexing is built into the primers; library preparation is done in a single tube with no ligation or tagmentation, and hands-on time drops without compromising coverage. Result: fast, robust workflows for viral surveillance (e.g. SARS-CoV-2), HLA typing or gene panels in microbiology and translational research, primarily on Illumina platforms. Fewer steps lower the risk of cross-contamination, make inter-site standardisation easier and simplify pooling thanks to integrated indexing.

- In Sanger/CE, NimaGen provides a reliable, cost-effective alternative to legacy consumables: BrightDye cycle-sequencing kits, ExS-Pure PCR clean-up, NimaPOP polymers, capillaries, buffers and ROX size standards compatible with Applied Biosystems 3130/3500/3730 sequencers. R&D, quality control and non-regulated diagnostic/LDT laboratories value lot-to-lot consistency, chromatogram quality and straightforward integration with existing instruments and software.

Backed by clear documentation, proven protocols and responsive support, NimaGen stands out for pragmatic thinking: solutions that save hours, reduce cost per sample and safeguard day-to-day results.

Nimagen products supplied by Dutscher

Frequently asked questions about the Nimagen brand

Are NimaGen CE (capillary electrophoresis) consumables compatible with my ABI instruments?
Yes, NimaPOP polymers, capillaries, buffers and ROX size standards are designed for Applied Biosystems 3130/3500/3730. Check your instrument model.

What does RC-PCR offer compared with conventional library prep methods?
Indexing is built into the primers: a single PCR, fewer steps, fewer touchpoints and lower error risk, notable time savings, with uniform coverage.

Which NGS platforms do EasySeq kits run on?
EasySeq RC-PCR solutions are designed primarily for Illumina systems. See the product pages for the full list of compatible instruments.

Are NimaGen reagents RUO or IVD?
The majority of catalogue references are intended for research use only (RUO). Refer to the product labelling and instructions for use for regulatory status and any exceptions.

Does RC-PCR help to reduce costs and waste?
Yes. Fewer steps and consumables per sample mean lower cost, faster workflows and less single-use plastic day to day.

What level of support is offered?
Detailed protocols, reference datasets and responsive technical support. Dutscher guides you through selection, installation and routine optimisation.