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Amido Black analysis:
detection and quantification of proteins

 

What is the Amido Black method?

 

 

Amido Black (Amido Black 10B) is an anionic dye used for the detection and quantification of proteins. It binds to proteins via electrostatic interactions with the basic residues in the protein chains. After staining, the proteins appear as coloured bands or patches, the intensity of which is proportional to the amount of protein present in the sample. It is a standard laboratory method for quality control, research and the analysis of the true protein content of milk.

 

NF V04-216 standard: the official reference for the method 
The Amido Black assay is governed by the NF V04-216 standard, which precisely defines the protocol for determining true protein content in milk using this colorimetric method. This standard sets out the conditions for sample preparation, colour development, rinsing and reading, thereby ensuring the reproducibility and reliability of the results obtained, regardless of which laboratory carries out the analysis.

 

A method validated by the CNIEL 
The Noir Amido technique has been validated by the CNIEL (National Inter-professional Centre for the Dairy Industry) for the analysis of the true protein content of cow’s, sheep’s and goat’s milk. This validation grants the method official recognition within the French dairy sector, making it a benchmark tool for inter-professional laboratories, dairies and milk quality control bodies. 
 

Applications

  • Protein analysis following electrophoresis
  • Quantification of total proteins
  • Assay of true proteins in cow’s, sheep’s and goat’s milk (NF V04-216 standard, CNIEL-validated method)
  • Laboratory quality control
  • Research and development

Advantages of the Noir Amido technique

  • A quick and simple method to implement
  • Good detection sensitivity
  • Controlled costs
  • Excellent reproducibility, compliant with standard NF V04-216
  • Compatible with many types of samples (milk, sera, protein extracts)

Procedure for the analysis

  1. Sample preparation
  2. Application to the test medium
  3. Staining with Amido Black
  4. Rinsing
  5. Reading and interpretation of results (spectrophotometry)


All the equipment required for the Noir Amido analysis

We supply all the equipment and consumables required to carry out the Noir Amido analysis in accordance with standard NF V04-216 and the method validated by the CNIEL:

  • Spectrophotometer for reading the results
  • Test tubes
  • Peristaltic pump for dosing and dispensing reagents
  • Noir Amido dye
  • Diluter
  • As well as all associated consumables and accessories


Your results

The results may be presented as a qualitative identification, a comparative assessment or a precise quantification of the protein content, depending on the requirements of the analysis and the provisions of standard NF V04-216.

 

 

Whether you are a multi-sector laboratory, a dairy or a quality control organisation, we can support you with reliable equipment that complies with the NF V04-216 standard and the CNIEL method, for the analysis of true proteins in cow’s, sheep’s and goat’s milk.

 

See V-730 LACTO V-ONE spectrophotometers for amido black